N-OCTANOYLSPHINGOSINE, D-ERYTHRO
C8 Ceramide
CAS: 74713-59-0
Molecular Formula: C26H51NO3
N-OCTANOYLSPHINGOSINE, D-ERYTHRO - Names and Identifiers
N-OCTANOYLSPHINGOSINE, D-ERYTHRO - Physico-chemical Properties
| Molecular Formula | C26H51NO3 |
| Molar Mass | 425.69 |
| Density | 0.9974 (rough estimate) |
| Melting Point | 68-70°C |
| Boling Point | 541.37°C (rough estimate) |
| Solubility | DMSO or ethanol: soluble |
| Appearance | waxy solid |
| Color | White |
| Storage Condition | −20°C |
| Stability | Temperature Sensitive |
| Refractive Index | 1.6000 (estimate) |
| In vitro study | C8-ceramide (3 μM; 48 hours) irreversibly reduces tumor-cell proliferation and induces morphological changes. C8-ceramide can induce necrosis-like cell death, but does not induce caspase-dependent cleavage of PARP (biochemical marker of apoptosis) in human cervical tumor cells. C8-ceramide may increase the endogenous ROS level (10-30 µM; 24 hours) by regulating the switch of SOD1 and SOD2, causing the anti-proliferation (10-50 µM; 24 hours), and consequently triggering the apoptosis (10-50 µM; 48 hours) of NSCLC H1299 cells. Cell Viability Assay Cell Line: CALO cells, INBL cells, HeLa cells Concentration: 3 μM Incubation Time: 48 hours Result: Markedly reduced the tumor cell number. Cell Proliferation Assay Cell Line: H1299 cells Concentration: 10 µM, 20 µM, 30 µM, 40 µM, 50 µM Incubation Time: 24 hours Result: Decreased the rate of cellular proliferation in a dose-dependent manner, with an IC 50 of 22.9 µM. Cell Cycle Analysis Cell Line: H1299 cells Concentration: 10 µM, 20 µM, 30 µM, 40 µM, 50 µM Incubation Time: 24 hours Result: Caused the G1 arrest. Apoptosis Analysis Cell Line: H1299 cells Concentration: 10 µM, 20 µM, 30 µM Incubation Time: 24 hours, 48 hours Result: Increased the level of cleaved caspase-3. |
| In vivo study | C8-ceramide (0.1 mg/kg; intranasal administration) induces more robust CD8 + and CD4 + T cell responses to viral infections in virus infected mice. Animal Model: C57BL/6 mice, with lymphocytic choriomeningitis virus infected Dosage: 0.1 mg/kg Administration: Intranasal administration Result: Increased the CD8 + T cell response to influenza in the lungs. |
N-OCTANOYLSPHINGOSINE, D-ERYTHRO - Risk and Safety
| Safety Description | 24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 3 |
| HS Code | 29225000 |
N-OCTANOYLSPHINGOSINE, D-ERYTHRO - Reference Information
| biological activity | C8-Ceramide (N-Octanoyl-D-erythro-sphingosine) is a cell-permeable endogenous ceramide analog. C8-Ceramide has Anti proliferative properties, can be used as a chemotherapeutic agent. C8-Ceramide can stimulate dendritic cells to promote T cell response to viral infection. C8-Ceramide slightly induced PKC activation in vitro. |
| Target | PKC apoptosis autophagy |
| In vitro studies | C8-ceramide (3 μm. C8-ceramide can industry necrosis-like cell death, but does not induce caspase-dependent cleavage of PARP (biochemical marker of apoptosis) in human obstetric tumor cells. C8-ceramide may increase the endogenous ROS level (10-30 µm; 24 hours) by regulating the switch of SOD1 and SOD2, causing the anti-proliferation (10-50 µM; 24 hours), and consequently triggering the apoptosis (10-50 µM; 48 hours) of NSCLC H1299 cells. Cell Viability Assay Cell Line: CALO cells, INBL cells, HeLa cells Concentration: 3 μM Incubation Time: 48 hours Result: Markedly reduced the tumor cell number. Cell Proliferation Assay Cell Line: H1299 cells Concentration: 10 µM, 20 µM, 30 µM, 40 µM, 50 µM Incubation Time: 24 hours Result: Decreased the rate of cellular proliferation in a dose-dependent manner with an IC 50 of 22.9 µM. Cell Cycle Analysis Cell Line: H1299 cells Concentration: 10 µM, 20 µM, 30 µM, 40 µM, 50 µM Incubation Time: 24 hours Result: Caused the G1 arrest. Apoptosis Analysis Cell Line: H1299 cells Concentration: 10 µM, 20 µM 30 µM Incubation Time: 24 hours, 48 hours Result: Increased the level of cleaved caspase-3. |
| Cell Line: | CALO cells, INBL cells, HeLa cells H1299 cells H1299 cells H1299 cells |
| Concentration: | 3 μM 10 µM, 20 µM, 30 µM, 40 µM, 50 µM 10 µM, 20 µM, 30 µM, 40 µM, 50 µM 10 µM, 20 µM, 30 µM |
| Incubation Time: | 48 hours 24 hours 24 hours 24 hours, 48 hours |
| Result: | Markedly reduced the tumor cell number. Decreased the rate of cellular proliferation in a dose-dependent manner, with an IC 50 of 22.9 µM. Caused the G1 arrest. Increased the level of cleaved caspase-3. Increased the CD8 + T cell response to influenza in the lungs. |
| in vivo study | C8-ceramide (0.1 mg/kg; Intra administration) induced more robust CD8 and CD4 T cell responses to viral infections in virus infected mice. Animal Model: C57BL/6 mice, with mathematical chronomenetitis virus infected docage: 0.1 mg/kg Administration: intransal administration Result: Increased the CD8 T cell response to influenza in the lungs. |
| Animal Model: | C57BL/6 mice, with lymphocytic choriomeningitis virus infected |
| Dosage: | 0.1 mg/kg |
| Administration: | Intranasal administration |
Last Update：2024-04-09 20:45:29
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Multiple Specifications
Product Name: C8 Ceramide Visit Supplier Webpage Request for quotationCAS: 74713-59-0
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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